biotinylated goat anti mouse immunoglobulin g secondary antibody  (Vector Laboratories)

Journal: Cancer Cell International

Article Title: Overexpression of microRNA-29b inhibits epithelial-mesenchymal transition and angiogenesis of colorectal cancer through the ETV4/ERK/EGFR axis

doi: 10.1186/s12935-020-01700-2

Figure Lengend Snippet: ETV4 promoted EGFR transcription to activate ERK signaling pathway. a Expression of EGFR mRNA and protein in cells detected by RT-qPCR and Western blot. b The binding of ETV4 to EGFR promoter in HEK293 and HCT116 cells detected by Luciferase assay. c The binding of ETV4 to EGFR promoter by CHIP. * p < 0.05 compared with the NC group, MUT, or IgG. Each experiment was repeated 3 times independently. Data between the two groups were compared by unpaired t -test

Article Snippet: After the sections were dried, they were incubated with the biotinylated secondary goat anti-mouse immunoglobulin G (IgG) (1: 1000, ab6789, Abcam) for 30 min at 37 °C.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Binding Assay, Luciferase